Figure 5.

EMSA assay of the NF-κB translocation to the nucleus in HUVECs treated with TSFZR75.30. Nuclear extracts from HUVECs untreated and treated with TNF, TSFMCF-7 or TSFZR75.30, were subjected to EMSA using a probe for NF-κB. Roman numbers indicate NFκB/DNA complexes. Numbers below the complexes correspond to relative intensity of complex III normalized against the control signal, are the mean of two individual experiments with independent cell cultures.