Informatic analyses with the program Image Master 2D Platinum™. 50 μg of total extracts were loaded and stained with the Deep-Purple fluorochrome™. The 1st dimension was resolved in a linear pH-range from 3-10 and in 10% acrylamide for the 2nd dimension. Control (C): left panels; TNF: central panels; TSFZR75.30: right panels. Lower panels represent an amplified view of the dotted rectangle in the upper panels. Patterns are the representative images of three individual experiments each performed in duplicate.
Montes-Sánchez et al. BMC Chemical Biology 2009 9:7 doi:10.1186/1472-6769-9-7