Table 1

DNA sequence of non-fluorescent clones chosen randomly from the library generated with COBARDE

Clone

sgGFP modified region

Codons deleted


Ile 129

Asp 130

Phe 131

Lys 132

Glu 133

Asp 134

Gly 135

Asn 136

Ile 137

Leu 138

Gly 139

His 140

Lys 141

Leu 142


Wt

0

1

ATC

TTC

GGC

AAC

10

2

ATC

GAC

TTC

AAG

GAA

GAC

GGC

AAC

ATC

CTG

GGA

CAC

CTG

1

3

ATC

GAC

TTC

AAC

GGA

CAC

AAA

CTG

6

4

ATC

TTC

AAG

AAC

ATC

CTG

GGA

CAC

CTG

5

5

ATC

GAC

TTC

AAG

GAA

GAC

GGC

AAC

CTG

5

6

ATC

AAC

ATC

GGA

CAC

CTG

8

7

ATC

TTC

GAG

GGC

CTG

9

8

ATC

GAC

GAC

AAC

CTG

9

9

ATC

GAC

AAG

GAA

GAC

GGC

AAC

GGA

AAA

CTG

4

10

ATC

GAC

TTC

AAG

AAC

CTG

GGA

CTG

6

11

ATC

GAC

GAA

GAC

ATC

9

12

ATC

GAC

GAC

GGC

ATC

CTG

GGA

7

13

ATC

GAC

TTC

AAG

GAA

GAC

GGC

AAC

CAC

CTG

4

14

ATC

GAC

AAG

GAA

GGC

AAC

ATC

CTG

CAC

AAA

CTG

3

15

ATC

GAC

AAG

GAA

GAC

CTG

8

16

ATC

GAC

GAA

GAC

GGC

ATC

CAC

CTG

6

17

ATC

GAC

AAG

GAA

GAC

AAC

8

18

ATC

GAC

AAC

AAA

CTG

9

19

ATC

GAC

TTC

GAC

GGC

ATC

CTG

GGA

AAA

CTG

4

20

ATC

GAC

TTC

AAG

GAC

CTG

8

21

ATC

GAC

TTC

GAA

GAC

GGC

AAC

GGA

CAC

AAA

CTG

3

22

ATC

GAC

TTC

AAC

CTG

AAA

CTG

7

23

ATC

AAG

GAC

CTG

10

24

ATC

TTC

AAG

GGC

AAC

9

25

ATC

TTC

GAA

GGC

ATC

CTG

CTG

7

26

ATC

GAC

TTC

GAA

GAC

GGC

AAC

GGA

CAC

AAA

CTG

3

27

ATC

GAC

TTC

GAA

GGC

CTG

8

28

ATC

G

TTC

GAA

GGC

AAC

CTG

CAC

CTG

6

29

ATC

GAC

AAG

GGC

AAC

ATC

GGA

CT

6

30

ATC

T

AAG

GAC

AAC

CTG

GGA

AAA

CTG

5

31

AC

GAC

ATC

CTG

GGA

CAC

AAA

CTG

6

32

ATC

GA

GGC

AAC

CTG

GGA

AAA

7

33

ATC

GAC

AAG

AAC

CTG

GA

8

oligo

TAG

CTG

AAG

TTC

CTT

CTG

CCG

TTG

TAG

GAC

CCT

GTG

TTT

GAC


Empty spaces represent deleted codons. Last row contains the oligonucleotide segment that was subjected to mutagenesis. Dots represent single nucleotide deletions. Nucleotide insertions are shown in gray letters.

Flores-Ramírez et al. BMC Chemical Biology 2007 7:1   doi:10.1186/1472-6769-7-1

Open Data