Oligonucleotides used for preparation of sgGFP mutants as described in M&M. For clear visualization, codons subjected to combinatorial deletion are alternatively labeled in bold and underlined. Asterisks represent nucleotide deletions. N represents an equimolar mixture of the four nucleotides and S a mixture of G and C. Restriction sites are double-underlined.
Flores-Ramírez et al. BMC Chemical Biology 2007 7:1 doi:10.1186/1472-6769-7-1