Table 1

Kinetic parameters for RNA/DNA unmodified hammerhead ribozyme derivatives.

Ribozyme

S-30

S-550


kmax (min-1)

"Kd" nM

kmax/"Kd" (min-1μM-1)

kmax (min-1)

"Kd" nM

kmax/"Kd" (min-1μM-1)


Rz-12/12

0.8 ± 0.1

7 ± 12

114 ± 200

0.7 ± 0.1

2600 ± 900

0.3 ± .2

Mz-12/12

0.22 ± 0.08

31 ± 6

7 ± 4

0.12 ± 0.01

240 ± 50

0.5 ± .2

Mrz-8/8

0.56 ± 0.06

210 ± 80

2.7 ± 1.3

0.005 ± 0.001

2200 ± 500

0.002 ± .001

Mrz-12/12

4.2 ± 0.3

75 ± 30

56 ± 26

1.6 ± 0.1

120 ± 50

13 ± 6

Rz-6/12

9 ± 1

400 ± 200

22 ± 13

0.6 ± 0.1

2800 ± 900

0.2 ± .1


Cleavage conditions; 37°C, pH 7.6, 10 mM MgCl2, ribozyme is in large excess over RNA substrates S-30 (30 nt) and S-550 (550 nt).

Hendry et al. BMC Chemical Biology 2004 4:1   doi:10.1186/1472-6769-4-1

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