Detection of T.C mismatch of 547 bp DNA fragment by using hydroxylamine / piperidine assay. The control trace (homoduplex, top) shows no cleavage peak and the mismatched DNA trace (heteroduplex, bottom) displays a strong cleavage peak of 5'FAM sequence at the mismatched C base. Note this is the second chance of detecting the mutation, the first being in Fig 2.
Bui et al. BMC Chemical Biology 2003 3:1 doi:10.1186/1472-6769-3-1