Table 2

1H et13C NMR data for the saponin 3-GlcA-28-AraRhaxyl-medicagenateaobtained in CD3OD at 298K and 500 MHz
13C ppm 1H ppm
Aglycone (medicagenic acid))
1 44.4 2.11 dd (14.6, 2.3); 1.25b
2 70.6 4.29 m
3 86.1 4.08 d (3.5)
4 53.0
5 52.8 1.59b
6 21.0 1.64b, 1.16b
7 33.0 1.76b, 1.54b
8 40.8
9 49.0 1.60b
10 37.1
11 24.3 2.01b, 1.94b
12 123.5 5.32 t (3.2)
13 144.6
14 42.8
15 28.4 1.67b, 1.11b
16 23.4 2.04b, 1.67b
17 47.5
18 42.1 2.93 dd (13.5, 4.3)
19 46.8 1.15b, 1.12b
20 31.4
21 34.3 1.39b, 1.22b
22 33.2 1.34b, 1.31b
23 181.4
24 13.1 1.40 s
25 16.9 1.24 s
26 17.4 0.81 s
27 25.9 1.16 s
28 177.5
29 33.1 0.91 s
30 23.6 0.95 s
GlcA
1 104.9 4.40 d (7.7)
2 74.5 3,27b
3 77,9 3.30 t (9.1)
4 73.0 3.48 t (9.1)
5 76.8 3.36 d (9.1)
6 173.2
Ara
1 93.6 5.69 d (3.5)
2 75.3 3.80 t (4.5)
3 70.5 3.90 m
4 66.7 3.84 m
5 62.9 3.91 m, 3.49 m
Rha
1 101.2 5.03 s
2 71.8 3.86b
3 70.8 3.47b
4 83.1 3.55 m
5 68.5 3.72 m
6 17.3 1.28 d (6.2)
Xyl
1 106.5 4.49 d (7.7)
2 75.5 3.19 dd (9.1, 5.2)
3 76.8 3.37 t ((9.1)
4 70.7 3.56 m
5 67.1 3.84b, 3.18 dd (11.5, 9.4)

a Italic letters indicate the multiplicity of the NMR peaks and J values are in parentheses (Hz).

b overlapping signals.

Da Silva et al.

Da Silva et al. BMC Chemical Biology 2012 12:3   doi:10.1186/1472-6769-12-3

Open Data