Table 3

GUS expression in response to different pH during co-cultivation on MSC containing GlA40 after transformation of leaf explants of different plant species using 1 ×109 cfu ml-1 A. tumefaciens

S. No.

Plant Species

pH

GUS Expression


(+) GlA40

(-) GlA40


1.

Nicotiana tabacum (tobacco)

5.2

+++++ (< 1 day)

+++++ (< 1 day)


5.6

+++++ (< 1 day)

+++++ (< 1 day)


5.9

+++++ (< 1 day)

+++++ (< 1 day)


7.0

-

-


2.

Podophyllum hexandrum (Indian may apple)

5.2

-

-


5.6

++++ (2 days)

-


5.9

+++ (2 days)

-


7.0

-

-


3.

Aloe vera (aloe)

5.2

+++ (1 day)

-


5.6

+++ (1 day)

-


5.9

-

-


7.0

-

-


4.

Lavendula officinalis (lavender)

5.2

+++ (1 day)

-


5.6

++++ (1 day)

-


5.9

+++ (1 day)

-


7.0

-

-


5.

Rosa sp. (Wild rose)

5.2

-

-


5.6

+++ (1 day)

+ (4 days)


5.9

+++ (1 day)

+ (5 days)


7.0

-

-


6.

Malus domestica (apple rootstock)

5.2

++++ (2 days)

+ (3 days)


5.6

++++ (2 days)

+ (3 days)


5.9

++++ (2 days)

+ (3 days)


7.0

-

-


7.

Dendrocalamus asper (bamboo)

5.2

+ (2 days)

+ (6 days)


5.6

+++ (1 day)

+ (3 days)


5.9

++ (1 day)

+ (4 days)


7.0

-

-


8.

Cynodon dactylon

(grass)

5.2

-

-


5.6

++ (1 day)

+ (5 days)


5.9

++ (1 day)

+ (3 days)


7.0

-

-


9.

Zea mays (maize)

5.2

+++ (1 day)

+ (3 days)


5.6

+++ (1 day)

+ (3 days)


5.9

++++ (1 day)

+ (5 days)


7.0

-

-


10.

Oryza sativa (rice)

5.2

++ (1 day)

+ (4 days)


5.6

+++ (1 day)

+ (2 days)


5.9

+++ (1 day)

+ (2 days)


7.0

-

-


11.

Aurocaria (gymnosperm)

5.2

-

-


5.6

++++ (3 days)

-


5.9

-

-


7.0

-

-


12.

Dryopteris (fern, pteridophyte)

5.2

++ (1 day)

+ (7 days)


5.6

+++ (1 day)

+ (3 days)


5.9

-

-


7.0

-

-


where + = one to five tiny blue spots; ++ = one to two large blue spots; +++ = small or faint blue patches; ++++ = large blue patch covering almost quarter of the leaf surface; +++++ = entire leaf showed distinct blue color and - = no GUS expression (Values in parenthesis represent the duration of co-cultivation)

Sandal et al. BMC Chemical Biology 2011 11:1   doi:10.1186/1472-6769-11-1

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