Effects of the CID755673 analogs on prostate cancer cell migration. The analogs inhibited wound healing in prostate cancer cells. DU145 cells (A) or PC3 cells (B) were grown to confluence in 6-well plates. The monolayer was wounded and imaged immediately. Cells were then treated with either vehicle (DMSO) or analogs at indicated concentration for 24 hours. Cells were then fixed and stained with 0.5% crystal violet. Percentage wound closure was calculated as an average of 9 determinations for each concentration/compound as described under "Methods." Data shown are the mean ± S.E.M. for three independent experiments. Statistical significance versus the DMSO control was determined by unpaired t-test in GraphPad Prism V. ns, not statistically significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.
LaValle et al. BMC Chemical Biology 2010 10:5 doi:10.1186/1472-6769-10-5