CID755673 and its analogs cause accumulation of cyclin D1 and cyclin D3. A, PC3 cells were treated with increasing concentrations of CID755673 for 48 hrs. Inhibitor and growth media were refreshed after 24 hrs. Western blots for cyclin D1 and cyclin D3 are shown. B, PC3 cells were treated with 25 μM CID755673, 10 μM kb-NB142-70, 10 μM kb-NB165-09, 1 μM kb-NB165-92, or 10 μM kb-NB184-02 for 48 hrs. Note that 1 μM kb-NB165-92 was used in this assay since this compound at 10 μM caused significant cell death. Inhibitors and growth media were refreshed after 24 hrs. Western blots for cyclin D1 and D3 are shown. GAPDH was used as a loading control.
LaValle et al. BMC Chemical Biology 2010 10:5 doi:10.1186/1472-6769-10-5