Figure 5.

Effect of triptolide on mRNA and protein expression of TLR 4 (mean ± S.D.). The mRNA expression of TLR 4 was measured by the mRNA quantity relative to the response to LPS activation (positive control) that was normalized to a value of 1.00; lower values represent greater inhibitory effects with 0.00 corresponding to a complete inhibition of the induced gene expression. The value of negative control (no induction) was normalized to 0.00. A. mRNA expression of TLR 4; B. Protein expression of TLR 4; C. Densitometric analysis for protein expression. * Significantly different from control (p < 0.05) ANOVA followed by LSD