Effect of triptolide on gene expression of COX-2, iNOS and chemokines in response to various TLR ligand activation in RAW macrophages. The effect of triptolide treatment (three replicates) on a specific gene expression was measured by the mRNA quantity relative to the response to ligand activation only (positive control) that was normalized to a value of 1.00; lower values represent greater inhibitory effects with 0.00 corresponding to a complete inhibition of the induced gene expression. The value of the negative control (no induction) was normalized to 0.00. Values are mean ± S.D. *, p < 0.05; (post-ANOVA comparison with Ligand-treated positive control). A. Effect of triptolide on mRNA levels of COX-2, iNOS and CCL3 following zymosan stimulation. B. Effect of triptolide on mRNA levels of COX-2, iNOS, and IRG-1 following Poly I:C stimulation. C. Effect of triptolide on mRNA levels of COX-2, iNOS, CCL3 and IRG-1 following LPS stimulation.
Premkumar et al. BMC Chemical Biology 2010 10:3 doi:10.1186/1472-6769-10-3