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Open Access Research article

Mitotic slippage in non-cancer cells induced by a microtubule disruptor, disorazole C1

Fengfeng L Xu1, Youssef Rbaibi1, Kirill Kiselyov1, John S Lazo2, Peter Wipf3 and William S Saunders1*

Author Affiliations

1 Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA

2 Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA

3 Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA

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BMC Chemical Biology 2010, 10:1  doi:10.1186/1472-6769-10-1

Published: 11 February 2010

Additional files

Additional file 1:

Figure S1. Structures of disorazole A1 and DZ.

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Additional file 2:

Figure S2. Proliferation assay of RPE-hTERT cells treated with DZ and VBL, indicating that DZ inhibited growth of RPE-hTERT cells.

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Additional file 3:

Supplemental Movie. Cells were treated with 10 nM of DZ for 16 hours before imaging. Cells escaped from mitotic arrest and formed abnormally shaped nuclei, indicating mitotic slippage had occurred.

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Additional file 4:

Figure S3. H2O2-treated cells demonstrating cytochrome C release retained normal microtubule structures. Left panel: α-tubulin staining. Right panel: cytochrome C staining.

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Additional file 5:

Figure S4. Only little or no PARP cleavage was observed in UP3 (primary cells) and HFF-hTERT (non-cancer cells) after DZ treatment. un: untreated.

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Additional file 6:

Figure S5. DNA fragmentation assay using untreated REP-hTERT cells and RPE-hTERT cells treated with DZ revealed little DNA laddering upon DZ treatment (left panel) while H2O2-treated RPE-hTERT cells demonstrated DNA laddering (right panel). An arrow indicates DNA ladder, a marker of apoptosis.

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