Table 2

Transgenic mitigation study:percent germination and transgenic segregation frequencies under non-competitive and competitive conditions.

Parent Populations

Progeny Populations: Competition

Progeny Populations: No Competition


Line

Seeds Plated

Germination (%)

Transgenic Ratio (%)

MS

Seeds Sown

Germination (%)

Transgenic Ratio (%)

MS

Seeds Sown

Germination (%)

Transgenic Ratio (%)

MS


BN

125

100

0

K

125

93.9

1.4

IJ

125

89.1

2.8

HI

BR

125

100

0

K

125

92.2

7.3

G

125

89.8

5.7

GH

GT1

125

80.7

55.1

C

125

89.9

38.5

D

125

88.6

36.8

D

GT5

125

79.3

63.9

BC

125

88.9

34

D

125

93.3

37.1

D

GT9

125

90.7

32.1

DE

125

90.7

10.3

FG

125

89.8

10.4

FG

TM(H)

125

86.4

100

A

125

81.6

77.7

ABC

125

82.6

88.3

AB

TM(B)

125

87.2

22.4

EF

125

84.3

0.4

JK

125

87.3

0.7

IJK


Percent germination and transgenic frequencies were calculated for the parental (BN: B. napus; BR: B. rapa; TM(H) and hybrid BC1/F2 populations (GT1, GT5, GT9, and TM) pre-season and post-harvest. Transgene persistence data reported here only applies to gene flow from GT hybrid plots and not TM hybrid or homozygous plots. Column MS represents transgenic ratio mean separation obtained by Fisher's LSD (P < 0.0001). Data was rank transformed and SAS software was used for data analysis.

Rose et al. BMC Biotechnology 2009 9:93   doi:10.1186/1472-6750-9-93

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