Table 4 |
|
|
Effect of expression system and additives on proteolytic stability of scFv AB28. |
|
|
scFv preparation |
ED50 |
|
|
|
|
scFv (tobacco) |
3,786.00 |
|
|
|
|
scFv (pea) |
1,209.00 |
|
|
|
|
scFv (tobacco) + 15% BSA |
3,053.00 |
|
|
|
|
scFv (tobacco) + wt pea seed extract |
83.24 |
|
|
|
|
AB28-pea seed extract |
33.47 |
|
|
|
|
ED50, effective dilution of chicken intestinal fluid leading to 50% loss of activity. |
|
|
Zimmermann et al. BMC Biotechnology 2009 9:79 doi:10.1186/1472-6750-9-79 |
|
