Research article
Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens
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* Corresponding author: Sergey M Kipriyanov s.kiprijanov@affitech.com
- † Equal contributors
1 Novoplant GmbH, Am Schwabeplan 1b, 06466 Gatersleben, Germany
2 Leibniz-Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, 06466 Gatersleben, Germany
3 Clinic for Poultry, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany
4 Current address : Affitech AS, Oslo Research Park, Gaustadalléen 21, 0349 Oslo, Norway
BMC Biotechnology 2009, 9:79 doi:10.1186/1472-6750-9-79
Published: 11 September 2009Additional files
Additional file 1:
Characterization of AB28-transgenic pea lines.: The data provided represent PCR and Western blot analyses of F0 and F1 transgenic pea plants.
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Additional file 2:
Analyses of independent AB28 F0 pea lines. The data provided represent a summary of basta selection, PCR and Western blot analyses of independent F0 transgenic pea lines.
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Additional file 3:
Analyses of F1 seeds derived from AB28 F0 pea line 9. The data provided represent a summary of PCR and Western blot analyses of F1 seeds derived from AB28 F0 pea line 9.
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Additional file 4:
Results of analyses of the progeny of F1 lines 9/9, 9/10, 9/11 and 9/13. The data provided represent a summary of Western blot analyses of F2 seeds derived from different AB28 F1 lines.
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Additional file 5:
Characterization of purified scFv AB28 produced in pea seeds. The data provided represent analysis of the molecular forms of pea-derived scFv and head-to-head comparison of the antigen-binding properties of the scFv AB28 preparations isolated either from the tobacco leaves or from the transgenic pea seeds.
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Additional file 6:
Preliminary ad libitum feeding experiment. The data provided represent analyses of the body weight and the feed consumption by chickens fed ad libitum with the fodder containing either transgenic or wt pea together with the assessment of shunning transgenic pea shred.
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Additional file 7:
Feed consumption and antibody uptake in chickens. The data provided represent measured average feed consumption, body weight gains and calculated antibody uptake by chickens in a preliminary feeding experiment.
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Additional file 8:
List of expressed recombinant antigens of Eimeria tenella, their gene accession numbers and used PCR primers. The file provides information about generation of recombinant antigens used in the present study.
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Additional file 9:
Characteristics of the binary vector used for pea transformation. The file provides information about the genetic elements included into the binary vector used for pea transformation.
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