Table 1

Lethal dose response data derived from topical bioassays of the parent M. anisopliae ARSEF2575, EVG016 and EVG017g strains with adult M. sanguinipes grasshoppers at 28°C.

M.a. strain

Assay

LD₅₀

95% CL

Slope (SE)

Chi Sqr

LD₉₅

95% CL

2575

1

799

63–1,722

1.46 (0.51)

0.04

10,599

4,415–36,196

2

1,815

1,174–3,042

1.60 (0.24)

1.55

19,503

9.279–68,978

mean (S.D.)

1,307 (718)

15,051 (6,296)

EVG016

1

25,453

19,600–41,000

3.73 (0.82)

0.75

70,257

50,534–138,856

2

19,758

14,000–2.7400

2.55 (0.37)

2.89

87,347

57,234–169,968

mean (S.D.)

22,605 (4,027)

78,802 (12,084)

EVG017g

1

8,939

4,425–13,194

2.50 (0.64)

0.71

40,787

25,601–127,114

2

14,007

4,365–25,838

1.62 (0.41)

2.35

145,180

71,373–765,860

mean (S.D.)

11,473

(3,584)

92,983 (73,817)

Units for LD and confidence levels: conidia/insect. Data are derived from two replicate bioassays using a total of 120–150 insects/bioassay.

de Crecy et al. BMC Biotechnology 2009 9:74   doi:10.1186/1472-6750-9-74