Table 3 |
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|
Sequence diversity of antibody fragments selected against four different antigens |
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|
Clone |
Family |
CDR3 |
Germline |
Amino acids differences from germline |
|
|
||||
|
VH gene |
||||
|
Aflatoxin C3 |
VH1 |
ADDYGSGSYGFDY |
IGHV1-3*01 (DP25) |
1 |
|
Aflatoxin C5 |
VH3 |
SRVGLWGPRYYYYYGMDVW |
IGHV3-23*04 (DP47) |
4 |
|
Aflatoxin D2 |
VH1 |
GGPLDY |
IGHV1-45*02 (DP4) |
5 |
|
Rabies D7 |
VH1 |
GGNFDY |
IGHV1-18*01 (DP14) |
2 |
|
Rabies B5 |
VH3 |
GYATFDY |
IGHV3-23*01 (DP47) |
7 |
|
Cobra D11 |
VH4 |
HGRDTSGYTMDYFDS |
IGHV4-59*07 (H4) |
14 |
|
Carcinoma C4 |
VH3 |
DRGKYPGDGMGV |
IGHV3-23*01 (DP47) |
6 |
|
VL gene |
||||
|
Aflatoxin C3 |
VK1 |
QQSYSTPYA |
IGKV1D-39*01 (DPK9) |
4 |
|
Aflatoxin C5 |
VL3 |
QVWDRDSRTIV |
IGLV3-9*01 (V2-6) |
16 |
|
Aflatoxin D2 |
VL2 |
SSYAGSNNLV |
IGLV2-8*01 (V1-2) |
3 |
|
Rabies D7 |
VL1 |
AAWDDSLSGPV |
IGLV1-47*01 (DPL3) |
2 |
|
Rabies B5 |
VK1 |
QQYSYNPYT |
IGKV1D-39*01 (DPK9) |
1 |
|
Cobra D11 |
VL6 |
QSYDSSNRV |
IGLV6-57*01 (V1-22) |
5 |
|
Carcinoma C4 |
VL1 |
AAWDDSLNGYV |
IGLV1-44*01 (DPL2) |
2 |
|
|
||||
|
* CDR3 sequence and V-gene segment usage for both heavy and light chains of the selected antibody are reported. Sequence analysis was done by Ig BLAST [36] and DNA Plot program [34] (shown in parenthesis). |
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|
Pansri et al. BMC Biotechnology 2009 9:6 doi:10.1186/1472-6750-9-6 |
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