Open Access Methodology article

Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

Antoine W Caron1*, Claire Nicolas1, Bruno Gaillet12, Ismaïla Ba1, Maxime Pinard1, Alain Garnier2, Bernard Massie134 and Rénald Gilbert15*

Author Affiliations

1 Institut de Recherche en Biotechnologie, Conseil National de Recherches du Canada, 6100 Royalmount Avenue, Montréal, QC H4P 2R2, Canada

2 Chemical Engineering Department, Université Laval, Québec, QC, G1K 7P4, Canada

3 Département de Microbiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montreal, QC, H3C 3J7, Canada

4 INRS-IAF, Université du Québec, Laval, QC, H7N 4Z3, Canada

5 Neuromuscular research group, Montreal Neurological Institute, Montreal, QC, H3A 2B4, Canada

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BMC Biotechnology 2009, 9:42  doi:10.1186/1472-6750-9-42

Published: 11 May 2009

Additional files

Additional File 1:

Identification of high-secreting CHO clones: comparison between surface detection by flow cytometry and FLSSM. Additional data showing labeling of CHO-IGF-E5 cells with the anti His Tag-FITC antibody followed by flow cytometry.

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