Effect of amine acetylation on polyplex dissociation by heparin competition. Complexes (N/P = 10) were prepared in PBS at a final siRNA concentration of 2 μg/mL, and were exposed to heparin for 60 min at 37°C. siRNA release from complexes was determined by PicoGreen fluorescence as in Figure 2. Data represent mean ± SEM (n = 5).
Waite et al. BMC Biotechnology 2009 9:38 doi:10.1186/1472-6750-9-38