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Open Access Research article

Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer

Amy Raymond1,2, Scott Lovell1,2, Don Lorimer1,2,3, John Walchli3,4, Mark Mixon3,4, Ellen Wallace1,3, Kaitlin Thompkins1,2, Kimberly Archer1,2, Alex Burgin1 and Lance Stewart1,2,3*

Author Affiliations

1 deCODE biostructures Inc, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA

2 Seattle Structural Genomics, Center for Infectious Disease, Bainbridge Island, WA 98110, USA

3 Accelerated Technologies, Center for Gene to 3D Structure, Bainbridge Island, WA 98110, USA

4 Emerald BioSystems Inc, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA

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BMC Biotechnology 2009, 9:37 doi:10.1186/1472-6750-9-37

Published: 21 April 2009

Additional files

Additional file 1:

Gene and protein sequences for all targets. Nucleic acid and amino acid sequences for all targets.

Format: DOC Size: 102KB Download file

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