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Open Access Research article

Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer

Amy Raymond12, Scott Lovell12, Don Lorimer123, John Walchli34, Mark Mixon34, Ellen Wallace13, Kaitlin Thompkins12, Kimberly Archer12, Alex Burgin1 and Lance Stewart123*

Author Affiliations

1 deCODE biostructures Inc, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA

2 Seattle Structural Genomics, Center for Infectious Disease, Bainbridge Island, WA 98110, USA

3 Accelerated Technologies, Center for Gene to 3D Structure, Bainbridge Island, WA 98110, USA

4 Emerald BioSystems Inc, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA

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BMC Biotechnology 2009, 9:37  doi:10.1186/1472-6750-9-37

Published: 21 April 2009

Abstract

Background

With the goal of improving yield and success rates of heterologous protein production for structural studies we have developed the database and algorithm software package Gene Composer. This freely available electronic tool facilitates the information-rich design of protein constructs and their engineered synthetic gene sequences, as detailed in the accompanying manuscript.

Results

In this report, we compare heterologous protein expression levels from native sequences to that of codon engineered synthetic gene constructs designed by Gene Composer. A test set of proteins including a human kinase (P38α), viral polymerase (HCV NS5B), and bacterial structural protein (FtsZ) were expressed in both E. coli and a cell-free wheat germ translation system. We also compare the protein expression levels in E. coli for a set of 11 different proteins with greatly varied G:C content and codon bias.

Conclusion

The results consistently demonstrate that protein yields from codon engineered Gene Composer designs are as good as or better than those achieved from the synonymous native genes. Moreover, structure guided N- and C-terminal deletion constructs designed with the aid of Gene Composer can lead to greater success in gene to structure work as exemplified by the X-ray crystallographic structure determination of FtsZ from Bacillus subtilis. These results validate the Gene Composer algorithms, and suggest that using a combination of synthetic gene and protein construct engineering tools can improve the economics of gene to structure research.