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Open Access Highly Accessed Methodology article

A multi-Fc-species system for recombinant antibody production

Sandrine Moutel14, Ahmed El Marjou24, Ole Vielemeyer24, Clément Nizak5, Philippe Benaroch34, Stefan Dübel6 and Franck Perez24*

Author Affiliations

1 Translational Research Department, 26 rue d'Ulm, F75248 Paris Cedex 05, France

2 CNRS UMR144, 26 rue d'Ulm, F75248 Paris Cedex 05, France

3 INSERM U653, 26 rue d'Ulm, F75248 Paris Cedex 05, France

4 Research Center, Institut Curie, 26 rue d'Ulm, F75248 Paris Cedex 05, France

5 CNRS, UMR 5588, Université Joseph Fourier -, BP 87, 140 avenue de la Physique, Domaine Universitaire, 38402 Saint Martin d'Hères Cedex, France

6 Dept. Biotechnology Technical, University of Braunschweig, Spielmannstr. 7, 38106 Braunschweig, Germany

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BMC Biotechnology 2009, 9:14  doi:10.1186/1472-6750-9-14

Published: 26 February 2009

Abstract

Background

Genomic, transcriptomic and proteomic projects often suffer from a lack of functional validation creating a strong demand for specific and versatile antibodies. Antibody phage display represents an attractive approach to select rapidly in vitro the equivalent of monoclonal antibodies, like single chain Fv antibodies, in an inexpensive and animal free way. However, so far, recombinant antibodies have not managed to impose themselves as efficient alternatives to natural antibodies.

Results

We developed a series of vectors that allow one to easily fuse single chain Fv antibodies to Fc domains of immunoglobulins, improving their sensitivity and facilitating their use. This series enables the fusion of single chain Fv antibodies with human, mouse or rabbit Fc so that a given antibody is no longer restricted to a particular species. This opens up unlimited multiplexing possibilities and gives additional value to recombinant antibodies. We also show that this multi-Fc species production system can be applied to natural monoclonal antibodies cloned as single chain Fv antibodies and we converted the widely used 9E10 mouse anti-Myc-tag antibody into a human and a rabbit antibody.

Conclusion

Altogether, this new expression system, that brings constant quality, sensitivity and unique versatility, will be important to broaden the use of recombinant and natural monoclonal antibodies both for laboratory and diagnosis use.