PCR amplification for single-site deletions. A) Agarose gel electrophoresis of the PCR reactions indicating the amplification efficiency. The names of the mutants are shown on the top of each lane. B) Transformation and mutation efficiency for VraRNHISD, VraRC5D and VraRN3D, cloned vraR genes with its N-terminal His tag removal, five residues from the C-terminus and three residues from the N-terminus deleted respectively.
Liu and Naismith BMC Biotechnology 2008 8:91 doi:10.1186/1472-6750-8-91