PCR amplification for single-site mutagenesis. A) Agarose gel electrophoresis of the PCR reactions indicating the amplification efficiency. The names of the mutants are shown on the top of each lane. B) Transformation and mutation efficiency of each reaction for 3026L/M, 3051L/M, 3327L/M, 3356L/M, FabH2111C/A, FabH2111C/S. Labels for the generated mutants are detailed in the text. 3026L/M(13) and 3026L/M(QC) are control PCR reactions using primer pairs designed as  and QuickChange™ protocol respectively.
Liu and Naismith BMC Biotechnology 2008 8:91 doi:10.1186/1472-6750-8-91