Table 2

Molecular Weight and pI for the proteins examined here.

Characteristics

GvpC

Tat

Rev

Nef1


pI

3.73

4.92

8.17

5.31

pI (Fusion proteins)

3.83

3.88

4.07

MW (Da)

42,392

5,743

9,313

24,733

Expected MW

48,117

51,687

67,106

Predicted MW

60,000

65,743

69,313

84,733


† Expected MW is calculated as GvpC + SIV insert; Tat, Rev and Nef1 respectively.

‡ Predicted MW is based on results for GvpC from wild type GV (NRC-1) where the protein band was indicated at ~ 60 kDa [18]. Accordingly, the MW for the recombinant proteins was calculated as a sum of the specific insert and the MW for the GvpC. Isoelectric points were calculated using the Web page for pI/MW calculation [42].

Sremac and Stuart BMC Biotechnology 2008 8:9   doi:10.1186/1472-6750-8-9

Open Data