Immunogen specific ELISA titrations of antibody elicited by rGVTat, Rev or Nef1 encoded sequences. Mice were initially immunized using isolated r-GV and subsequently boosted as specified in the Methods. The three dimensional graphs display the temporal profiles of antigen specific antibody responses to the SIV peptide expressed within the GvpC protein. For Tat, all post-4 week samples exhibit a titer of ≥ 1:1,280 while for Rev, the maximal titer, 1:320 occurs in the week 2 post second booster sera. For Nef1, the highest titer, 1:320, is evident in sera from week 8 post second booster and by the 12 week time point, only sera from the animals immunized with r-GV expressing Rev retained a peptide specific titer.
Sremac and Stuart BMC Biotechnology 2008 8:9 doi:10.1186/1472-6750-8-9