Verification of recombinant GvpC protein formation and incorporation into functional gas vesicles. Western blots were used to assess putative recombinant gas vesicles (Panel A) and cell lysates (Panel B) for the presence of r-GvpC protein. Here samples are resolved in 8–16% SDS-PAGE gels, immunoblotted and probed with rabbit anti-GvpC antibody. The size range of the protein standards (kDa) is indicated at the left and the recombinant GvpC protein bands are identified at the right.
Sremac and Stuart BMC Biotechnology 2008 8:9 doi:10.1186/1472-6750-8-9