Spheroplast formation by the gas vesicle deficient strain SD109 results in a distinctive, spherical morphology. With the addition of spheroplasting solution and 0.5 M EDTA, the rod shape halobacteria cells (Panel A) rapidly changes into the transformable spheroplast form (Panel B) exhibiting a characteristic change in morphology detectible microscopically. Spheroplasts can then be transformed with the recombinant plasmids pMS104D::tat,::rev or ::nef1 carrying the native leftward operon of the gas vesicle gene cluster and the rightward operon carrying an inserted SIV fragment of interest. Original magnifications 1,000 ×.
Sremac and Stuart BMC Biotechnology 2008 8:9 doi:10.1186/1472-6750-8-9