Recombinant expression of MAβ(1–42)E22G. (A) SDS-PAGE using a 16.5% Tris-Tricine gel at 4°C. Lane 1: Marker. 2: MAβ(1–42). 3: MAβ(1–42)E22G. (B) Elution profile of analytical SEC of purified MAβ(1–42)E22G. (C) 15N HSQC NMR spectra of MAβ(1–42) (blue) and MAβ(1–42)E22G (red) at 5°C at 800 MHz. Resonances of MAβ(1–42) that are lost or displaced as a consequence of the E22G mutation are indicated. Assignments were obtained from literature spectra [35,52].
Macao et al. BMC Biotechnology 2008 8:82 doi:10.1186/1472-6750-8-82