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Open Access Methodology article

Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin

Xiu-Mei Cai1, Hai-Long Xie2, Ming-Zhu Liu1 and Xi-Liang Zha1*

Author Affiliations

1 Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, PR China

2 Institute of Cancer Research, South China University, Hengyang, PR China

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BMC Biotechnology 2008, 8:74  doi:10.1186/1472-6750-8-74

Published: 18 September 2008

Abstract

Background

Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth inhibition was obtained. This study was to further investigate whether the EGF-targeted phagemid particles carrying siRNA would be a promising tool for anti-cancer siRNA delivery.

Results

We found that pSi4.1-siFAK phagemid particles could significantly inhibit the expression of focal adhesion kinase in the HCPT-treated cells. Moreover, we also observed that the particles could potently suppress cell growth and cell invasion.

Conclusion

These results indicated that EGF-targeted phagemid particles might be a promising tool for anti-cancer siRNA delivery in the presence of HCPT.