Table 4 |
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Specific activities, production and iron quantitation assay of purified CrHydA1 anaerobically expressed in S. oneidensis AS52A1N strain grown with DMSO, TMAO or thiosulfate as substrates for anaerobic respiration. |
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Anaerobic respiration substrate used for cell growth |
Activity of HydA1 μmol H2 mg-1 min-1 |
Atoms of Fe/molecule of protein |
Production of HydA1 protein μg/L of culture(OD600 = 1) |
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|
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TMAO 10 mM |
705 ± 18 |
6.4 ± 0.5 |
400 ± 20 |
|
Thiosulfate 20 mM |
695 ± 33 |
5.89 ± 0.32 |
420 ± 35 |
|
DMSO 10 mM |
740 ± 56 |
6.08 ± 0.12 |
500 ± 28 |
|
|
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Values are: result ± standard deviation (at least three replicates of all experiments). |
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Sybirna et al. BMC Biotechnology 2008 8:73 doi:10.1186/1472-6750-8-73 |
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