Table 1

Timetable of progressive deprivation of serum over the 6-day culture period of chondrocyte/agarose constructs.

FBS

ITS

Ascorbic acid

Hepes buffer


Day 0

10%

-

-

10 mM

Day 1

10%

-

-

10 mM

Day 2

5%

-

5 ng/mL

20 mM

Day 3

5%

1%

10 ng/mL

20 mM

Day 4

1%

1%

15 ng/mL

30 mM

Day 5

-

1%

20 ng/mL

30 mM

Day 6

-

1%

20 ng/mL

30 mM


Timetable of progressive substitution of ITS for serum in the culture medium of the chondrocyte/agarose constructs, over the 6-day culture period preceding compression. The concentrations of ascorbic acid and Hepes were progressively increased to allow recovery of the cells after their extraction.

Bougault et al. BMC Biotechnology 2008 8:71   doi:10.1186/1472-6750-8-71

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