|
Timetable of progressive deprivation of serum over the 6-day culture period of chondrocyte/agarose constructs. |
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| FBS |
ITS |
Ascorbic acid |
Hepes buffer |
|
|
|
||||
| Day 0 |
10% |
- |
- |
10 mM |
| Day 1 |
10% |
- |
- |
10 mM |
| Day 2 |
5% |
- |
5 ng/mL |
20 mM |
| Day 3 |
5% |
1% |
10 ng/mL |
20 mM |
| Day 4 |
1% |
1% |
15 ng/mL |
30 mM |
| Day 5 |
- |
1% |
20 ng/mL |
30 mM |
| Day 6 |
- |
1% |
20 ng/mL |
30 mM |
|
Timetable of progressive substitution of ITS for serum in the culture medium of the chondrocyte/agarose constructs, over the 6-day culture period preceding compression. The concentrations of ascorbic acid and Hepes were progressively increased to allow recovery of the cells after their extraction. | ||||
Bougault et al. BMC Biotechnology 2008 8:71 doi:10.1186/1472-6750-8-71 |
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