Analysis of the human COL2A1 gene promoter activity in response to compression. Analysis of the activity of the COL2A1 gene promoter in chondrocytes embedded in agarose gels immediately after the cell/agarose constructs were subjected to dynamic loading at 0.2 Hz for 30 min. A decrease in COL2A1 promoter activity in response to dynamic loading is observed in comparison with the control constructs that remained uncompressed. Data are reported as means of three independent experiments with the standard deviations. The dashed line indicates the uncompressed control levels. ** indicates significant difference between the compressed samples and the controls, p < 0.05.
Bougault et al. BMC Biotechnology 2008 8:71 doi:10.1186/1472-6750-8-71