Analysis of the molecular responses of chondrocytes cultured in agarose gels to dynamic compression. Analysis of the responsiveness of chondrocytes in agarose gels immediately (0 min) or 15 min and 60 min after being subjected to dynamic loading at 0.33 Hz for 30 min. Control correspond to uncompressed cell/agarose constructs. A) Measurement of MAP kinases activation using Western-blotting analysis. A representative blot with quantification of phosphorylated ERK1/2 and p38 is shown. Mechanically-induced phosphorylation was normalized to uncompressed controls, as referred to the dashed line. Transient phosphorylation of ERK1/2 and p38 kinase is observed in response to dynamic compression. B) cFos and cJun mRNA levels are up-regulated in response to dynamic compression, as measured immediately after 30 min compression (time = 0 min after compression). Data are reported as means of three independent experiments with the standard deviations. The dashed line indicates the uncompressed control levels. * indicates significant difference between the compressed samples and the controls, p < 0.10. The electropherogram at the top shows the integrity of RNA used for RT-PCR analysis, as assessed by capillary electrophoresis with the Agilent 2100 Bioanalyzer.
Bougault et al. BMC Biotechnology 2008 8:71 doi:10.1186/1472-6750-8-71