Table 4

Comparison of HPV16 viral load and physical state determined by Southern hybridisation with NA6 qPCR analysis of HPV16 E2 and E6 copy numbers in cervical keratinocyte cell lines.

Cell line

Ploidy

Southern analysis Copy numbers per cell

NA6 qPCR analysis


E2 copies per diploid genome

E6 copies per diploid genome

Viral load per cell

Ratio of E2/E6 (range)


C33A

Near triploid

HPV16 negative

0

0

0

0.00

W12.Ser1p16

Diploid

Episomal (approx 100 copies)

148

143

143

1.04 (0.76–1.32)

W12.Ser1p57

Tetraploid

Integrated (approx 1 copy)

0.2

0.3

1

0.72 (0.57–3.82)

SiHa

Triploid

Integrated (2–3 copies)

0.7

3.4

5

0.20 (0.11–0.24)

CaSki

Tetraploid

Integrated (>1000 copies)

2,272

869

1,738

2.61 (1.37–3.46)


Values less than 1 were rounded to the nearest whole number when calculating viral load per cell. E2/E6 ratio values were calculated from the qPCR readings for E2 and E6 and rounded to the nearest two decimal places.

Roberts et al. BMC Biotechnology 2008 8:57   doi:10.1186/1472-6750-8-57

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