Generation of mean NA6 calibration curve for E2, E6 amd HMBS quantification. Panels A – C show representative data from the four replicate PCR runs using serially diluted NA6 as template (2 ng – 2 fg), from which the mean calibration curve for viral quantification was generated. The different curves correspond to different starting amounts of template. The fluorescence thresholds are indicated by the lines of crosses running horizontally. Panel A shows E2 log transformed data from replicate 1 qPCR run, panel B shows HMBS log transformed data from replicate 3 qPCR run, panel C shows E6 log transformed data from replicate 2 qPCR run. Panel D demonstrates the tight correlation between the data points generated for the E2 amplicon at each template concentration in the four replicate runs. Similar findings were made for the E6 and HMBS amplicons. Panel E shows the mean calibration curves for E2, E6 and HMBS used for viral gene copy number quantification, together with the respective line equations.
Roberts et al. BMC Biotechnology 2008 8:57 doi:10.1186/1472-6750-8-57