Email updates

Keep up to date with the latest news and content from BMC Biotechnology and BioMed Central.

Open Access Highly Accessed Research article

Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR

Ian Roberts*, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff and Nicholas Coleman

BMC Biotechnology 2008, 8:57  doi:10.1186/1472-6750-8-57

PubMed Commons is an experimental system of commenting on PubMed abstracts, introduced in October 2013. Comments are displayed on the abstract page, but during the initial closed pilot, only registered users can read or post comments. Any researcher who is listed as an author of an article indexed by PubMed is entitled to participate in the pilot. If you would like to participate and need an invitation, please email info@biomedcentral.com, giving the PubMed ID of an article on which you are an author. For more information, see the PubMed Commons FAQ.

Equation 2 is incorrect

Bob Rutledge   (2008-10-23 16:06)  Natural Resources Canada

Equation 2 should read:

E = 10-Slope - 1

It is also important to note that it is essential to fix the fluorescence threshold (Ft) to a single value across all runs, in order to avoid artificial quantitative variances.

The mathematics of standard curve construction and evaluation of quantitative variance generated by standard curves are addressed in detail in an earlier study:

Rutledge RG, Côté C: Mathematics of quantitative kinetic PCR and the application of standard curves.

Nucleic Acids Res 2003, 31(16):e93.

Competing interests

I have no competing interests.

top

Post a comment