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Open Access Highly Accessed Research article

Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR

Ian Roberts*, Grace Ng, Nicola Foster, Margaret Stanley, Michael T Herdman, Mark R Pett, Andrew Teschendorff and Nicholas Coleman

BMC Biotechnology 2008, 8:57  doi:10.1186/1472-6750-8-57

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Equation 2 is incorrect

Bob Rutledge   (2008-10-23 16:06)  Natural Resources Canada

Equation 2 should read:

E = 10-Slope - 1

It is also important to note that it is essential to fix the fluorescence threshold (Ft) to a single value across all runs, in order to avoid artificial quantitative variances.

The mathematics of standard curve construction and evaluation of quantitative variance generated by standard curves are addressed in detail in an earlier study:

Rutledge RG, Côté C: Mathematics of quantitative kinetic PCR and the application of standard curves.

Nucleic Acids Res 2003, 31(16):e93.

Competing interests

I have no competing interests.


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