Table 2

Summary of transgenic animals producing BChE/hSA fusion protein

Transgenic animal
Animal ID
Generation
Sex
Transgene copy number
Integration site
Expression in milk (g/liter)*

Mouse
307-1
F0
F
~40
ND
0.27

307-1A2
F1
F
1–2
ND
0.40

307-1A7
F1
F
8–10
ND
0.41

307-1A7A2
F2
F
8–10
ND
0.24

307-1A7A4
F2
F
1–2
ND
0.41

Goat
2176
F0
F
18–28
2
0.06

2177
F0
F
6–10
1–2
1.10

2178
F0
F
18–28
2–3
0.07

2229
F0
F
10–14
1–2
0.07

2231
F0
F
10–14
1–2
0.04

2232
F0
F
10–14
1–2
0.09

2233
F0
F
10–14
1–2
0.08

2234
F0
F
10–14
1–2
0.35

2236
F0
F
10–14
1–2
0.10

2237
F0
F
10–14
1–2
0.23

2238
F0
F
10–14
1–2
0.09

2239
F0
F
10–14
1–2
0.11

*The concentrations for expression of BChE/hSA, estimated by densitometry where 720 units = 1 mg of purified plasma huBChE. [29], were the average of two independent assays. ND = not determined.

Huang et al. BMC Biotechnology 2008 8:50   doi:10.1186/1472-6750-8-50