Additional file 1.

Derivation of the two sigmoidal functions describing PCR amplification. A Microsoft Word summary of the rearrangements and substitutions used for conversion of the classic Boltzmann four parameter sigmoid function into a form in which PCR amplification can be modeled, based upon amplification dynamics as described by ΔE and Emax (equation 3).

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Rutledge and Stewart BMC Biotechnology 2008 8:47   doi:10.1186/1472-6750-8-47