Optical calibrations of five reaction formulations supplemented with various quantities of SYBR Green I. Spreadsheet summary of the lambda gDNA (primer pair K7B-K12) optical calibrations conducted for the five reaction formulations used for LRE quantification in this study. Based on data presented in Figure 2, these formulations were designed to assess the impact of SYBR Green I quantity, the highest supplemented quantity (2.0X) estimated to be > 10X than that present in these three commercial formulations. Each row of values was derived from an individual amplification run (see additional file 3 for more details). (A) QuantiTect 0X SYBR Green I. (B) QuantiTect 0.2X SYBR Green I. (C) DyNAmo 0.5X SYBR Green I. (D) DyNAmo 1.5X SYBR Green I. (E) FullVelocity 2.0X SYBR Green I. Lam: lambda gDNA quantity in femtograms, QT: QuantiTect, DyNa: DyNAmo; FV: FullVelocity, SG: SYBR Green I, SD: standard deviation, CV: coefficient of variation (SD/Average × 100%).
Rutledge and Stewart BMC Biotechnology 2008 8:47 doi:10.1186/1472-6750-8-47