F0 plots for the amplification profiles presented in Figure 2. Following LRE analysis, individual fluorescence readings were converted into target quantity using equation 6 and the resulting values plotted back against the fluorescence readings. Numerical summaries of the F0 values encompassed by the LRE window (designated by red circles) are provided as inlays. The high level of similarity of F0 values within the LRE window is reflected by their respective CV values that range from +/-0.04% to +/-2.19%, illustrating the remarkable level of precision that can be achieved with LRE-based sigmoidal modeling. These plots also illustrate the rapid divergence in the F0 value generated by cycles within the upper region of these amplification profiles, due to loss of conformity with the LRE model.
Rutledge and Stewart BMC Biotechnology 2008 8:47 doi:10.1186/1472-6750-8-47