Additional file 1.

2D gel of potato (cv. Desiree) tuber proteins in patatin hpRNAi and WT plants. (A) 2D gel PI 4–7 of potato tuber protein of WT plants. (B) Patatin spots boxed in gel (A). (C) 2D gel PI 4–7 (the same area as (B)) of potato tuber proteins of patatin hpRNAi line 4. Patatin variants were almost completely suppressed in line 4, when compared to WT plants. Protein was extracted from potato tuber. 200 μg of protein extracts was separated by isoelectric focusing IPG pH 4–7 in the first dimension, and by 12.5% SDS/PAGE in the second dimension. The proteins were stained with silver nitrate.

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Kim et al. BMC Biotechnology 2008 8:36   doi:10.1186/1472-6750-8-36