|
Results of targeting experiments with the pHPRT PURO SV40 vector in S-phase cell synchronized hTERT transfected fibroblasts. |
||||||
| Exp |
# of cells |
PUROr+ |
8AGr+ |
6TGr+ |
PCR + |
Karyotype |
|
|
||||||
| 1 |
107 cells |
NC* |
1 |
1 |
1 |
Abnormal |
| 2 |
107 cells |
160 |
2 |
2 |
2 |
ND** |
| 3 |
107 cells |
170 |
7 |
7 |
6 |
ND** |
| 4 |
107 cells |
132 |
6 |
6 |
6 |
ND** |
| 5 |
107 cells |
122 |
3 |
3 |
2 |
ND** |
| 6 |
107 cells |
221 |
1 |
1 |
1 |
Normal |
| 7 |
107 cells |
152 |
0 |
0 |
0 |
ND** |
| 8 |
107 cells |
410 |
1 |
1 |
1 |
Normal |
|
*NC = not counted, **ND = not done | ||||||
Meehan et al. BMC Biotechnology 2008 8:31 doi:10.1186/1472-6750-8-31 |
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