The influence of D-glucose on the eGFP expression in E. coli JM109 with the plasmids pWA21, pWA73.1 and pWA64.1. The cells were grown for 2 h at 37°C in LB supplemented with D-glucose as indicated, then shifted to 30°C and induced with 0.2% (w/v) L-rhamnose and the fluorescence intensity was measured. Intensity after 2, 4, 6, 8 and 24 h are shown in (A) fluorescence per 0.01 OD600 and in (B) fluorescence per 1 ml culture. Values shown are the averages of three independent experiments, for the measuring points at 24 h the standard deviation is indicated.
Wegerer et al. BMC Biotechnology 2008 8:2 doi:10.1186/1472-6750-8-2