Plasmid stability after 48 h in liquid culture without antibiotic selection. Cells were grown at 37°C in LB supplemented with 0.2% (w/v) L-rhamnose for 24 h, starting from this culture fresh medium was inoculated, and again incubated for 24 h under the same conditions, which roughly matches 50 generations. The percentage of cells without plasmid or loss of fluorescence are shown, the values are the averages of three independent experiments.
Wegerer et al. BMC Biotechnology 2008 8:2 doi:10.1186/1472-6750-8-2