Figure 4.

Plasmid stability after 48 h in liquid culture without antibiotic selection. Cells were grown at 37°C in LB supplemented with 0.2% (w/v) L-rhamnose for 24 h, starting from this culture fresh medium was inoculated, and again incubated for 24 h under the same conditions, which roughly matches 50 generations. The percentage of cells without plasmid or loss of fluorescence are shown, the values are the averages of three independent experiments.