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Open Access Software

Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes

Fernando Lopes Pinto1, Håkan Svensson2 and Peter Lindblad1*

Author Affiliations

1 Department of Photochemistry and Molecular Science, The Ångström Laboratories, Uppsala University, Box 523, SE-75120, Uppsala, Sweden

2 Department of Molecular Evolution, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18, SE-75236, Uppsala, Sweden

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BMC Biotechnology 2007, 7:73  doi:10.1186/1472-6750-7-73

Published: 25 October 2007

Abstract

Background

Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination.

Results

The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are: 1) suitable for a specific organism, and 2) compatible with other oligonucleotides to be used in the experimental procedures.

Conclusion

This new web tool allows scientists to easily and rapidly obtain suitable tags for RT-PCR experiments, and is available at http://www.egs.uu.se/software/webtag/ webcite.