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Open Access Software

Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes

Fernando Lopes Pinto1, Håkan Svensson2 and Peter Lindblad1*

Author Affiliations

1 Department of Photochemistry and Molecular Science, The Ångström Laboratories, Uppsala University, Box 523, SE-75120, Uppsala, Sweden

2 Department of Molecular Evolution, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18, SE-75236, Uppsala, Sweden

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BMC Biotechnology 2007, 7:73  doi:10.1186/1472-6750-7-73

Published: 25 October 2007



Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination.


The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are: 1) suitable for a specific organism, and 2) compatible with other oligonucleotides to be used in the experimental procedures.


This new web tool allows scientists to easily and rapidly obtain suitable tags for RT-PCR experiments, and is available at webcite.