Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes
- Equal contributors
1 Department of Photochemistry and Molecular Science, The Ångström Laboratories, Uppsala University, Box 523, SE-75120, Uppsala, Sweden
2 Department of Molecular Evolution, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18, SE-75236, Uppsala, Sweden
BMC Biotechnology 2007, 7:73 doi:10.1186/1472-6750-7-73Published: 25 October 2007
Webtag is a tool providing oligonucleotide sequences (usually called tags or anchors) that are absent from a specified genome. These tags/anchors can be appended to gene specific primers for reverse transcriptase polymerase chain reaction experiments, circumventing genomic DNA contamination.
The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are: 1) suitable for a specific organism, and 2) compatible with other oligonucleotides to be used in the experimental procedures.