Research article
Engineering proteinase K using machine learning and synthetic genes
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* Corresponding author: Jeremy Minshull jminshull@dna20.com
1 Department of Computer Science, University of California, Santa Cruz, CA 95064 USA
2 DNA 2.0, 1430 O'Brien Drive, Suite E, Menlo Park, CA 94025, USA
BMC Biotechnology 2007, 7:16 doi:10.1186/1472-6750-7-16
Published: 26 March 2007Additional files
Additional File 1:
Supporting Material. Contains DNA and amino acid sequence of proteinase K, legends for structure figures (additional files 3, 4 and 5) and details of machine learning methods.
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Additional File 2:
Table 2. Contains sequence and activity information for all variants tested in this study.
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Additional File 3:
Supporting Material Figure 2. Positions of amino acid substitutions mapped onto the structure of proteinase K. The image should be opened using Swiss Protein Data Bank Viewer [64].
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Additional File 4:
Supporting Material Figure 3. Positions of amino acid substitutions mapped onto the structure of proteinase K.
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Additional File 5:
Supporting Material Figure 4. Positions of amino acid substitutions mapped onto the structure of proteinase K.
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