Quantification of internalized TAT-SA in living HeLa cells. (A) Quantitative analysis of the colocalization between both TAT-SA-A488 and TRITC-labeled transferrin and TRITC-labeled dextran (10 kD) at 15 min post transduction. (B) The relative fluorescence intensity of internalized TAT-SA-A488 was measured in cells treated with cytochalasin D (cytD), amiloride (ami) or nocodazole (noco) at 4 h post transduction. Control cells (C) were untreated. The fluorescence intensity data was collected from multiple series of cells by confocal microcopy and processed with the 3D LSM program.
Rinne et al. BMC Biotechnology 2007 7:1 doi:10.1186/1472-6750-7-1