Localization of internalized TAT-SA in HeLa cells by immunoelectron microscopy at 4 h post transduction. Intracellular TAT-SA was detected with pre-embedding immunolabeling technique, in which anti-SA staining was followed by silver-enhanced nanogold and gold-toning treatments. Localization of SA was shown as dark spots (arrow heads) within (A) cytoplasmic vesicular structures and further (B) in the nucleus of cells (nu). Scale bars, 1 μm.
Rinne et al. BMC Biotechnology 2007 7:1 doi:10.1186/1472-6750-7-1